Method

Injections of the vascular system were made both with the coloured rubber latex preparation supplied by Turtox Biological Supply Houses, and with oil paints mixed with turpentine. Latex was found to be admirable for making solid preparations of larger vascular spaces, such as the principal venous sinuses; it was injected through the foot, and also into the arterial system through the ventricle, after the animal had been anaesthetised by slowly adding small quantities of 70% alcohol to sea-water. Latex was found less convenient for demonstrating the finer details of the peripheral parts of the vascular system, owing to the thin, rather inelastic character of the walls of the vessels. For this purpose turpentine-bound oil colours were employed, and were injected in several ways—through the ventricle, into the pedal sinus, or directly into the rectal sinus. Oil paint was found to provide a beautiful injecting mass, by which the smallest features of the peripheral vascular system could be shown up; it has the double