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Volume 87, 1959
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A Note on the Spermiogenesis of Peripatoides novae-zealandiae

*

In 1912, Montgomery investigated the spermatogenesis of Peripatus, and claimed that there was complete rejection of the mitochondria during the metamorphosis of spermatid into spermatozoon. This would then be the only case known where the middle piece was not formed of mitochondria, in flagellate spermiogenesis.

In 1925, the present author studied some specimens of Peripatus (Peripatopsis) capensis from the Cape of Good Hope, and stated that while most of the mitochondria were undoubtedly rejected, a small portion still remained, and this took part in the formation of the middle-piece.

Recently, the spermiogenesis of the New Zealand Peripatoides has been studied with the light microscope, as a preliminary to the preparation of material for electron microscopy.

As in the Cape Peripatus, most of the mitochondria are rejected. In Fig. 4, the mitochondria have run together to form a ball (mr) which is peeled off the ripe spermatozoon. The latter has proportionately a very large nucleus, and a small middle piece (mp) as also occurs in P. capensis Between the nucleus and the middle piece is a more darkly stainable area (cx) in which could be one of three things—the head centriole, the centriole adjunct (post-nuclear body), or the combined head centriole with the centriole adjunct. It is not possible to settle this matter by means of the light microscope.

In Fig. 1 is a young metamorphosing spermatid with its posterior region towards the observer, the nucleus being at a lower level than the parts marked (c), (g), (mp), which have been drawn in as they came into focus. The flagellum (f) has grown out from the centriole (c), the main mass of mitochondria (mr) is ready for agglutinization prior to rejection, but at (mp) is a grouped collection of seven equal sized bodies which, with the powers of the light microscope can only be assumed to be mitochondria. It is these small spheres which unite to form the middle piece (mp) in Text-figs. 2 and 3. In P. capensis the granules which run together to form the middle piece are more numerous and finer. It will be clear that the centriole in Text-fig. 1 could be included within the middle piece, or above the middle piece in Figs. 2 and 3, or it could have flattened to form the body (cx) in Text-fig. 4. This can only be decided by electron microscopy, since the cells are so small. In Fig. 5 the upper end of the male genital tube is shown, the parts (i) containing spermatogonia and spermatocytes, (j) metamorphosing spermatids, and (k) the vas deferens with ripe spermatozoa.

Of about a dozen specimens of Peripatoides collected by splitting rotting logs near Lower Hutt, nine were males.

The author thanks Professor Richardson for various facilities and Mr. Graeme Ramsay for help in collecting. The osmium tetroxide was provided by the Irish Medical Research Council, Dublin.

[Footnote] * Professor of Zoology and Comparative Anatomy, Trinity College, Dublin, Ireland; Honorary Member of the Royal Society of New Zealand.

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Figs. 1–5. —For description see text. a, acrosome; c, centriole; cx, centriole + adjunct ? f, flagellum; g, Golgi apparatus (acroblast or dictyosome); gr, Golgi remnant; i, j, k, parts of male genital tract; mp, middle piece; mr, mitochondrial reject; n, nucleus.

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References

Afzelius, B. A., 1954. The Fine Structure of the Sea Urchin Spermatozoa, as revealed by the Electron Microscope Zeit. f. Zellforsch. Mikr. Anat., 42, p. 134.

Beams, H. W., Tahmisian, T. N., Devine, R. L., Anderson, E., 1956. Phase Contrast and Electron Microscope Studies on the Dictyosome and Acroblast (Golgi bodies) in the Male Germ Cells of the Cricket. Jour. Roy. Micr. Soc., 76.

Gatenby, J. Bronte., 1925. A Reinvestigation of the Spermatogenesis of Peripatus capensis. Quart. Journ. Micr. Sci.

—1931. Notes on the Post-Nuclear Acrosome Seal Granules and Vacuome of Desmognathus fusca. Jour. Morph. and Physiol., 51, p. 597.

—1931. The Post-Nuclear Granule in Anasa tristis. Amer. Jour. Anat., 48, p. 7.

Gatenby, J. Bronte, Tahmisian, T. N., Devine, R. L., Beams, H. W., 1958. The Orthopteron Acrosome, an Electron Microscope Study. La Celule (in press).

Gatenby, J. Bronte, and Tahmisian, T. N., 1958. Centriole Adjunct, Centrioles, Mitochondria, and Ergastoplasm in Orthopteran Spermatogenesis. La Cellule (in press).

Montgomery, Th, 1912. Complete Discharge of the Mitochondria from the Spermatozoon of Peripatus. Biol. Bull., 22, p. 56.

De Robertis, E., and Raffo, H. F., 1957. Submicroscopic Organisation of Mitochondrial Body and Cytoplasmic Structures of Insect Testis. Exper. Cell. Res., 12, p. 66.

Professor J. Bronte Gatenby,


Trinity College,
Dublin,
Ireland