
Crossing Experiments
Some closely controlled experiments were carried out at Christchurch and at Cass, where plants of the four different species were used to assess relative amounts of fruit set by female and hermaphrodite plants upon artifical crossing. Selfing of hermophrodites and cross pollination of hermaphrodites and females were used in these intraspecific crosses. No adequate figures for statistical treatment were obtained and no seed could be germinated to test viability. All flower heads used were bagged prior to opening of flowers, and care was taken to avoid accidental crosses. In all cases females set fruit in a high percentage of flowers, but very few attempts at crossing and selfing of hermaphrodites were successful. Enough evidence was gathered to show that P. “snow tussock”, P. “short tussock” and P. prostrata (the two latter more rarely) can all set fruit through self pollination of hermaphrodite flowers, but it is obvious that this is less efficacious than cross pollination of females. Cross pollination of emasculated hermaphrodites also results in a low percentage of fruit set in P. “snow tussock”.. Upon selfing, only a few fruit per head were set at Christchurch on P. “snow tussock” hermaphrodites but P. traversii hermaphrodites at Cass showed higher self fertility (8–10%). Artificial crosses with hermaphrodites thus resulted in fruit set comparable with that in natural conditions. Dr. E. Godley (pers comm.) has verified self fertility of hermaphrodites for P. traversii.

These experiments in Pimelea show that, in fact, not much more than half the amount of genic material supplied to a new generation comes from the hermaphrodites in P. “short tussock” and P. “snow tussock”, since hermaphrodite fruit set, whether due to selfing, or cross fertilization, is very low in them. Hermaphrodite fruit set is only about 4–5% of the total fruit set by females in natural populations of these two taxa. In P. prostrata the percentage fruit set of hermaphrodites as compared with females is also low—about 10%, but in P. traversii it is much higher —just less than 50%. The systems of inheritance of sex may differ between P. traversii and the other species but it is possible for them all to have a genetic basis. Cruickshank (1953) discussing Tasmanian species of Pimelea stated that “All species examined have fertile pollen, and there is no evidence of any apomixis in the genus.” The specimens from Cass were tested for apomixis by bagging (and emasculation of hermaphrodites) but no fruit was set by unpollinated flowers. All the species had apparently fertile pollen. Examination was made of pollen stained with cotton blue in lactophenol, and in each species the grains were full. Only a small percentage of pollen was deformed, abnormally small or empty.
